Бесплатный автореферат и диссертация по биологии на тему

Выявление и характеристика ДНК-связывающих белков, взаимодействующих in vitro и in vivo с хроматин-зависимым регуляторным участком ДНК мышиного mist

ВАК РФ 03.00.03, Молекулярная биология

Содержание диссертации, кандидата биологических наук, Прохорчук, Анна Валерьевна

Введение.

1.1. Метилирование генома эукариот.

1.1.1. Влияние метилирования ДНК на структуру хроматина in vivo u in vitro. v 'f.

1.1.2. Участие метилирования ДНК в регуляциц транскрипции генов.

1.1.3. Участие метилирования ДНК в процессах репликации и рекомбинации.

1.1.4. Защита последовательностей от de novo метилирования.

1.1.5. Геномный импринтинг.

1.1.6. Подавление активности гена ДНК метилтрансферазы летально для эмбрионов мыши.

1.2. Участие метилирования ДНК в возникновении и развитии опухолевых заболеваний.

1.3. Белки, связывающиеся с последовательностями ДНК, содержащими метилированные CpG-napbi.

1.3.1. Белки, участвующие в подавлении транскрипции генов, взаимодействуют с гистон деацетилазами.

1.4. Мышиный ген mtsl.

1.4.1. Белки, связывающие микросателлитные последовательности ДНК.

Заключение Диссертация по теме "Молекулярная биология", Прохорчук, Анна Валерьевна

Выводы

1. Делеция микросателлитной последовательности ( с координатами +804,+863 ) из первого интрона гена mtsl приводит к пятикратному понижению уровня транскрипции гена репортера при стабильной трансфекции клетки линии KCMJI-100.

2. Было показано, что микросателлитная последовательность, расположенная в области гиперчувствительности к обработке панкреатической эндонуклеазой ДНКазой1 (ДГУ2 ), связывает in vitro белок с молекулярной массой 40кДа .

3. Картирование участков гиперчувствительных к обработке микрококовой нуклеазой показало, что в области ДГУ2 нарушена нуклеосомная структура.

4. Каизо, белок вовлеченный в кадхерин-катениновую систему, специфично связывается с метилированной последовательностью ДНК из области ДГУ2.

Библиография Диссертация по биологии, кандидата биологических наук, Прохорчук, Анна Валерьевна, Москва

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