Бесплатный автореферат и диссертация по биологии на тему

Конструирование рекомбинантных плазмид, содержащих ген фибера вируса ССЯ-76 и изучение иммунного ответа лабораторных животных на введение этих плазмид

ВАК РФ 03.00.03, Молекулярная биология

Введение Диссертация по биологии, на тему "Конструирование рекомбинантных плазмид, содержащих ген фибера вируса ССЯ-76 и изучение иммунного ответа лабораторных животных на введение этих плазмид"

специальность 03.00.03 - молекулярная биология)

Диссертация на соискание ученой степени кандидата биологических наук научный.руководитель: доктор биологических наук, профессор, Б.С. Народицкий

МОСКВА 2 0 00 г.

ОГЛАВЛЕНИЕ.

Принятые сокращения. б

ВВЕДЕНИЕ.б

Заключение Диссертация по теме "Молекулярная биология", Уласов, Илья Валентинович

ВЫВОДЫ.

1. Впервые был получен набор рекомбинантных плазмид, содержащих, ген фибера вируса ССЯ-7 6 под контролем различных промоторов.

С помощью метода направленного мутагенеза были получены рекомбинатные плазмиды, содержащие нуклеотидные замены в вариабельной области гена фибера.

2. Впервые показано, что инъекция рекомбинантными плазмидами, содержащими ген фибера ССЯ-7 6 белым мышам (генетическая иммунизация), вызывает образование клеточного и гуморального иммунного ответа.

3. Разработаны схемы генетической иммунизации лабораторных животных рекомбинантными плазмидами, содержащими ген фибера вируса ССЯ-7 6.

4. Установлено наличие вируснейтрализующих антител (титр 4-51од2)в сыворотках крови лабораторных животных, подвергнутых генетической иммунизации рекомбинантными плазмидами.

5. Впервые проведен синтез изолированного белка фибера вируса ССЯ-7 6 в бакуловирусной системе, а также в культуре клеток млекопитающих и птиц, определена молекулярная масса рекомбинатного белка и изучены его антигенные свойства.

Библиография Диссертация по биологии, кандидата биологических наук, Уласов, Илья Валентинович, Москва

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