Бесплатный автореферат и диссертация по биологии на тему

Множественные формы протеинкиназ из клеток асцитной карциномы Эрлиха

ВАК РФ 03.00.04, Биохимия

Содержание диссертации, кандидата биологических наук, Токмаков, Александр Александрович

ВВЕДЕНИЕ.

ОБЗОР ЛИТЕРАТУРЫ.

1. сАМР-зависимые протеинкиназы.

2. сАМР-связыващие белки.

3. сШР-не зависимые протеинкиназы.

4. Активность протеинкиназ в клеточном цикле.

5. Активность протеинкиназ в трансформированной ткани молочной железы.7.

ЭКОПЕШ/ГЕНТАЛЬНАЯ ЧАСТЬ

Материалы.

Методы.

1. Культивирование клеток асцитной карциномы Эрлиха.

2. Фракционирование клеток асцитной карциномы Эрлиха методом седиментации в градиенте плотности сахарозы.

3. Определение S - фазы в клеточном цикле клеток Эрлиха.

4. Выделение и фракционирование протеинкиназ.

5. Определение активности протеинкиназ.

6. Определение сАМР-связыващей способности.

7. Выделение ингибитора каталитической активности сАМР-зависимых протеинкиназ.

8. Получение дефосфоршшрованного казеина.

9. Получение препаратов гистонов.

10. Определение молекулярного веса ферментов методом гельюильтрации.

11. Определение концентрации белка.

Библиография Диссертация по биологии, кандидата биологических наук, Токмаков, Александр Александрович, Ленинград

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