Бесплатный автореферат и диссертация по биологии на тему

Функции цистеин-богатых белков гордеивирусов

ВАК РФ 03.00.06, Вирусология

Содержание диссертации, кандидата биологических наук, Елина, Наталия Эдгардовна

ОГЛАВЛЕНИЕ.

СПИСОК СОКРАЩЕНИЙ.

ВВЕДЕНИЕ.

ОБЗОР ЛИТЕРАТУРЫ.

Взаимодействия растений с патогенами.

Умолкание генов.

Умолкание генов - механизм регуляции, характерный для эукариот.

Инициация ЯБ, функции днРНК.

Системное распространение ЯЭ.

Стадии ПТУ Г.

Общая схема деградации РНК в ходе ЯБ.

Клеточные белки, принимающие участие в ЯБ.

Роль ЯБ у различных организмов.

Супрессия умолкания генов вирус-специфическими белками.

Супрессия ЯБ - свойство вирусов, инфицирующих различные организмы.

Вирусы растений, способные подавлять ПТУ Г.

Обнаружение вирусных супрессоров ПТУГ.

Супрессор ПТУГ потивирусов.

Супрессоры ПТУГ кукумовирусов.

Супрессоры ПТУГ полеровирусов.

Супрессоры ПТУГ клостеровирусов.

Супрессор ПТУГ пеклу вирусов.

Супрессор ПТУГ Х-вируса картофеля.

Супрессоры ПТУГ томбусвирусов.

Белок оболочки - супрессор ПТУГ кармовирусов.

Супрессоры ПТУГ геминивирусов.

Супрессоры ПТУГ тоспо- и тенуивирусов.

Разнообразие супрессоров ПТУГ вирусов растений.

Супрессия ПТУГ и дальний транспорт вирусов.

Гордеивирусы.

Организация генома представителей рода Hordeivirus.

Цистеин-богатые белки yb гордеивирусов.

МАТЕРИАЛЫ И МЕТОДЫ.

Реактивы.

Ферменты и наборы.

Векторы для клонирования и бактериальные штаммы.

Трансформация бактериальных клеток Е. coli плазмидной ДНК.

Выделение плазмидной ДНК из клеток Е. coli.

Экспрессия рекомбинантных белков в клетках Е. coli.

Трансформация бактериальных клеток A. tumefaciens плазмидной ДНК.

Выделение плазмидной ДНК из клеток А. tumefaciens.

Электрофорез в агарозном геле.

Извлечение фрагментов ДНК из агарозы.

Расщепление ДНК рестрикционными эндонуклеазами.

Дефосфорилирование плазмидной ДНК.

Затупление концов фрагментов ДНК.

Лигирование фрагментов.

Секвенирование ДНК.

Амплификация фрагментов ДНК с помощью полимеразной цепной реакции (ПЦР).^

Транскрипция in vitro.

Иммунодетекция белков.

Приготовление тотальных препаратов РНК.

Блот-гибридизация РНК.

Растительный материал.

Инокуляция растений.

Агроинфильтрация.

Детекция GFP в растениях N. benthamiana.

Иммуно-ферментный анализ.

Получение рекомбинантных плазмид.

РЕЗУЛЬТАТЫ.

Изучение влияния экспрессии белка yb ПЛВМ на развитие инфекции

ВШМЯ.

Замена белка yb ВШМЯ на соответствующий белок ПЛВМ приводит к фенотипу "recovery".

Изучение влияния экспрессии гена yb ПЛВМ на накопление вирусных белков и РНК ВШМЯ.

Развитие вирусной инфекции ВШМЯ, несущего делецию гена yb.

Белок He-Pro А-вируса картофеля комплементирует функцию дальнего транспорта белка yb ВШМЯ.

Белок Нс-Рго усиливает симптомы инфекции ВШМЯ.

Белок Нс-Рго подавляет развитие фенотипа "recovery".

Экспрессия белков yb ВШМЯ и ПЛВМ в геноме Х-вируса картофеля.

Способность белков yb ВШМЯ и ПЛВМ подавлять ПТУГ при перекрестной защите».

Способность белков yb ВШМЯ и ПЛВМ подавлять ПТУГ при временной экспрессии генов в клетках N. benthamiana.

Мутагенез белка yb ПЛВМ.

Экспрессия мутантных белков yb ПЛВМ в геноме Х-вируса картофеля. 110 Изучение влияния мутаций в белке yb ПЛВМ на его способность подавлять ПТУГ.

ОБСУЖДЕНИЕ.

ВЫВОДЫ.

Заключение Диссертация по теме "Вирусология", Елина, Наталия Эдгардовна

выводы

1. Показано, что белок уЬ ПЛВМ, как и белок уЬ ВШМЯ, осуществляет регуляцию экспрессии вирусных БО и ТБГ1, а также синтеза вирусных РНК.

2. Установлено, что белок уЬ ВШМЯ является фактором дальнего транспорта в N. ЬеШкатгапа и по-разному осуществляет регуляцию экспрессии вирусных БО и ТБГ1 в однодольном и двудольном растениях-хозяевах.

3. Показана способность белка уЬ ПЛВМ усиливать проявление симптомов близкородственного вируса ВШМЯ, а также способность белков уЬ ПЛВМ и ВШМЯ увеличивать патогенность неродственного Х-вируса картофеля.

4. Доказана способность цистеин-богатых белков уЬ гордеивирусов ВШМЯ и ПЛВМ подавлять посттранскрипционное умолкание генов.

5. Методом сайт-направленного мутагенеза картированы последовательности белка уЬ ПЛВМ, необходимые для супрессии посттранскрипционного умолкания генов.

БЛАГОДАРНОСТИ

В заключение хочу выразить искреннюю благодарность Сергею Юрьевичу Морозову и Андрею Геннадьевичу Соловьеву - моим первым учителям и научным руководителям за неоценимую помощь, поддержку и внимание. Хочу также поблагодарить заведующего кафедрой вирусологии, академика Атабекова Иосифа Григорьевича за предоставленную возможность выполнить данную работу. Выражаю признательность Евгению Ивановичу Савенкову, Наталии Олеговне Калининой, Татьяне Николаевне Ерохиной, Виктору Константиновичу Новикову, Андрею Геннадьевичу Клюшину, Светлане Дмитриевне Зверевой, Дарье Викторовне Ракитиной, Нине Иосифовне Луховицкой, Андрею Александровичу Замятнину, Марине Владимировне Архипенко и Герхильд Юттнер за оказанную помощью в работе. Благодарю также наших иностранных коллег проф. Болкомба, проф. Валконена, проф. Джексона, д-ра Меритса, д-ра Шимана за предоставленные материалы. Большое спасибо моим преподавателям и всем сотрудникам кафедры вирусологии, которые помогали мне все эти годы.

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