Бесплатный автореферат и диссертация по биологии на тему

Детекция и изучение свойств гидрофобного белка ТБГЗ, кодируемого тройным блоком генов гордеивирусов

ВАК РФ 03.00.06, Вирусология

Содержание диссертации, кандидата биологических наук, Строганова, Татьяна Андреевна

Список сокращений.

Введение.

Обзор литературы.

Транспорт вирусов в растениях.

Строение плазмодесм.

Транспорт вирусов в растениях.

Вирусы, содержащие тройной блок генов.

Современная классификация вирусов.

Таксономическое положение вирусов, кодирующих тройной блок генов

Белки, принимающие участие в репликации.

Белки тройного блока генов.

Экспрессия белков тройного блока генов.

Белки ТБГ1.

Белки ТБГ2 и ТБГЗ.

Взаимодействие белков тройного блока генов.

Функциональные гомологи транспортных белков ТБГ.

Транспорт ТБГ-содержащих вирусов.

Локализация транспортных белков вирусов растений.

Локализация ТБГ белков ТБГ-со держащих вирусов растений.

Локализация транспортных белков других вирусов растений.

Материалы и методы.

Результаты.

Обсуждение.

Выводы.

Заключение Диссертация по теме "Вирусология", Строганова, Татьяна Андреевна

выводы

Показано, что при временной экспрессии в клетках растений ТБГЗ ПЛВМ сшитый с GFP локализуется во внутриклеточных включениях, расположенных на периферии клетки. Показано, что консервативная последовательность центрального гидрофильного участка белка ТБГЗ необходима для такой внутриклеточной локализации.

Получен клон-продуцент Е. coli, экспрессирующий белок ТБГЗ, сшитый с GFP. Проведены выделение и очистка препаративных количеств белка.

Получены моноклональные антитела к гидрофильным участкам белка ТБГЗ и GFP и определена их специфичность. Показана способность моноклональных антител реагировать с белком ТБГЗ, экспрессируемым в растениях.

Методом биохимического фракционирования белок ТБГЗ сшитый с GFP обнаружен в мембранной фракции тканей растений.

Показано, что центральный гидрофильный участок ТБГЗ ПЛВМ способен специфически взаимодействовать с N-терминальным районом белка ТБГ1 ПЛВМ.

Библиография Диссертация по биологии, кандидата биологических наук, Строганова, Татьяна Андреевна, Москва

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